Cryo-EM reconstruction of MAP7 83-134, bound to the microtubule
Ferro LS, Fang Q, Eshun-Wilson L, Fernandes J, Jack A, Farrell DP, Golcuk M, Huijben T, Costa K, Gur M, DiMaio F, Nogales E, Yildiz A
Science (2022) 375 pp. 326-331 [ DOI: doi:10.1126/science.abf6154 Pubmed: 35050657 ]
Cryo-EM reconstruction of Kinesin-1 and MAP7, bound to the microtubule
Ferro LS, Fang Q, Eshun-Wilson L, Fernandes J, Jack A, Farrell DP, Golcuk M, Huijben T, Costa K, Gur M, DiMaio F, Nogales E, Yildiz A
Science (2022) 375 pp. 326-331 [ DOI: doi:10.1126/science.abf6154 Pubmed: 35050657 ]
Cryo-EM structure of MAP7 MTBD and microtubule-associated protein tau, bound to the microtubule
Ferro LS, Fang Q, Eshun-Wilson L, Fernandes J, Jack A, Farrell DP, Golcuk M, Huijben T, Costa K, Gur M, DiMaio F, Nogales E, Yildiz A
Science (2022) 375 pp. 326-331 [ DOI: doi:10.1126/science.abf6154 Pubmed: 35050657 ]
Cryo-EM structure of full-length MAP7 bound to the microtubule
Resolution: 3.3 Å
EM Method: Single-particle
Fitted PDBs: 7sgs
Q-score: 0.5
Ferro LS, Fang Q, Eshun-Wilson L, Fernandes J, Jack A, Farrell DP, Golcuk M, Huijben T, Costa K, Gur M, DiMaio F, Nogales E, Yildiz A
Science (2022) 375 pp. 326-331 [ DOI: doi:10.1126/science.abf6154 Pubmed: 35050657 ]
- Magnesium ion (24 Da, Ligand)
- Guanosine-5'-triphosphate (523 Da, Ligand)
- Ensconsin (84 kDa, Protein from Homo sapiens)
- Tubulin alpha-1b chain (50 kDa, Protein from Sus scrofa)
- Guanosine-5'-diphosphate (443 Da, Ligand)
- Tubulin beta chain (49 kDa, Protein from Sus scrofa)
- Ternary complex of alpha-beta tubulin with microtubule-assosiated protein 7 (Complex from Sus scrofa)
13pf E254N microtubule from recombinant human tubulin decorated with EB3
LaFrance BJ, Roostalu J, Henkin G, Greber BJ, Zhang R, Normanno D, McCollum CO, Surrey T, Nogales E
PNAS (2022) 119 [ Pubmed: 34996871 DOI: doi:10.1073/pnas.2114994119 ]
- Human tubulin alpha-1b chain (tuba1b) with e254n mutation (Protein from Homo sapiens)
- Human tubulin beta-3 chain (tubb3) (Protein from Homo sapiens)
- Human microtubule end-binding protein 3 (eb3) (Protein from Homo sapiens)
- 13pf e254n microtubule decorated with eb3 from recombinant human tubulin (102 kDa, Complex from Homo sapiens)
Undecorated 13pf wildtype microtubule from recombinant human tubulin
Resolution: 3.8 Å
EM Method: Helical reconstruction
Fitted PDBs: 7sj7
Q-score: 0.452
LaFrance BJ, Roostalu J, Henkin G, Greber BJ, Zhang R, Normanno D, McCollum CO, Surrey T, Nogales E
PNAS (2022) 119 [ Pubmed: 34996871 DOI: doi:10.1073/pnas.2114994119 ]
- Magnesium ion (24 Da, Ligand)
- Tubulin beta-3 chain (51 kDa, Protein from Homo sapiens)
- Guanosine-5'-triphosphate (523 Da, Ligand)
- 13pf wildtype undecorated microtubule from recombinant human tubulin (102 kDa, Complex from Homo sapiens)
- Tubulin alpha-1b chain (51 kDa, Protein from Homo sapiens)
- Guanosine-5'-diphosphate (443 Da, Ligand)
13pf wildtype microtubule from recombinant human tubulin decorated with kinesin
Resolution: 3.6 Å
EM Method: Helical reconstruction
Fitted PDBs: 7sj8
Q-score: 0.428
LaFrance BJ, Roostalu J, Henkin G, Greber BJ, Zhang R, Normanno D, McCollum CO, Surrey T, Nogales E
PNAS (2022) 119 [ Pubmed: 34996871 DOI: doi:10.1073/pnas.2114994119 ]
- Magnesium ion (24 Da, Ligand)
- Tubulin beta-3 chain (51 kDa, Protein from Homo sapiens)
- Guanosine-5'-triphosphate (523 Da, Ligand)
- Tubulin alpha-1b chain (51 kDa, Protein from Homo sapiens)
- Guanosine-5'-diphosphate (443 Da, Ligand)
- 13pf wildtype microtubule from recombinant human tubulin and decorated with kinesin (102 kDa, Complex from Homo sapiens)
Undecorated 13pf E254A microtubule from recombinant human tubulin
LaFrance BJ, Roostalu J, Henkin G, Greber BJ, Zhang R, Normanno D, McCollum CO, Surrey T, Nogales E
PNAS (2022) 119 [ Pubmed: 34996871 DOI: doi:10.1073/pnas.2114994119 ]
13pf E254A microtubule from recombinant human tubulin decorated with EB3
Resolution: 3.8 Å
EM Method: Helical reconstruction
Fitted PDBs: 7sj9
Q-score: 0.441
LaFrance BJ, Roostalu J, Henkin G, Greber BJ, Zhang R, Normanno D, McCollum CO, Surrey T, Nogales E
PNAS (2022) 119 [ Pubmed: 34996871 DOI: doi:10.1073/pnas.2114994119 ]
- Tubulin beta-3 chain (51 kDa, Protein from Homo sapiens)
- Magnesium ion (24 Da, Ligand)
- Guanosine-5'-triphosphate (523 Da, Ligand)
- 13pf e254a microtubule decorated with eb3 (102 kDa, Complex from Homo sapiens)
- Microtubule-associated protein rp/eb family member 3 (32 kDa, Protein from Homo sapiens)
- Tubulin alpha-1b chain (50 kDa, Protein from Homo sapiens)
Undecorated 13pf E254N microtubule from recombinant human tubulin
Resolution: 3.8 Å
EM Method: Helical reconstruction
Fitted PDBs: 7sja
Q-score: 0.391
LaFrance BJ, Roostalu J, Henkin G, Greber BJ, Zhang R, Normanno D, McCollum CO, Surrey T, Nogales E
PNAS (2022) 119 [ Pubmed: 34996871 DOI: doi:10.1073/pnas.2114994119 ]
- Tubulin beta-3 chain (51 kDa, Protein from Homo sapiens)
- Magnesium ion (24 Da, Ligand)
- Guanosine-5'-triphosphate (523 Da, Ligand)
- Tubulin alpha-1b chain (51 kDa, Protein from Homo sapiens)
- Undecorated 13pf e254n microtubule from recombinant human tubulin (102 kDa, Complex from Homo sapiens)